Appendix: Protein Cleavage Sites

Table 1: Protein Cleavage Sites
表１：タンパク質開裂部位

name
(名称)		 recog seq
(認識配列) 		 comments
(コメント)
acid  D'P  pH 2.5, mild acid hydrolysis 
armillaria mellea protease  'K 
chymotrypsin  [FWY]' 
clostripain  R' 
cyanogen bromide  M' 
endopeptidase LysC  K' 
hydroxylamine  N'G  2M hydroxylamine, pH 9.0 
NBS(1)  '[WY]  N-bromo-succinimide, short incu-bation 
NBS(2)  '[HWY]  N-bromo-succinimide, long incu-bation 
NTCB  'C  2-nitro-5-thiobenzoic acid + Ni 
pancreatic elastase  '[AGSV] 
pepsin  '[FWY] 
proendopeptidase  P' 
thermolysin  '[ILV] 
trypsin  [KR]' 
V8(1)  E'  staphylococcal protease V8, ammonium acetate, pH 4 
V8(2)  '[DE]  staphylococcal protease V8,phosphate buffer, pH 7.8